PEROXIDASES ACTIVITY IN THE BARLEY CARYOPSES AFTER CRYOPRESERVATION

Monica Şipoş, Alina Dora Samuel, Gheorghe Bandici

ABSTRACT

Our research has aimed at tracing the peroxidase activity (PA) – during the first 44 hours of germination – in the barley caryopses (Hordeum vulgare L.) that have been frozen in liquid nitrogen (LN)(-196ºC) over variable periods of time (5 minutes, 1 hour, 1 day, 1 week or 1 month). After submersing the barley caryopses for 5 minutes or 1 hour in LN - with relation to the control lot represented by caryopses that were not subject to the treatment with LN - PA was particularly characterized by inhibitions.

The cryopreservation of the barley grains for 1 day has determined statistically insignificant stimulations of the PA after 20, 30 and 44 hours of germination. The cryopreservation for a longer period of time, 1 week or 1 month has proved to be the one that had best stimulated the PA of the barley caryopses; statistical relevance having the stimulations at 30 hours of germination, as well as those at 44 hours, in the case of the experimental variant of 1 month in LN.