THE REACTIVITY OF CYMBIDIUM HYBRIDUM PROTOCORMS VITROCULTIVATED UNDER WHITE FLUORESCENT

OR NATURAL LIGHT ILLUMINATION

 

Blidar Cristian Felix, Cachiţă-Cosma Dorina, Bandici Gheorghe Emil,

Radoveţ-Salinschi Dorina, Pop Liviu

 

 

The present paper offer new data about the reaction of the Cymbidium hybridum protocorms in the conditions of its vitrocultivation in mono- or bilayer system substrates: solid (agarized), respectively submersed under liquid culture medium, or in bilayer culture medium system – agarized on the basis of recipient, which was covered by a liquid stratum of culture medium, amplaced up to the protocorms; a last variant consisted in protocorms which were put on a filter paper-bridge, wich was submersed in the liquid culture medium. The vitrocultures were illuminated with fluorescent or natural light. After 90 days of vitroculture, the proliferation of the protocorm was maximum at the variant in which the protocorms were cultured submersed on the liquid medium, and were illuminated with white fluorescent light. But, the same variant, illuminated with natural light established the most economic protocorms proliferation, in the absence of the electric energy consumation, in which the number of neoprotocorms – generated per a bottle – in this last case was only with 1,7% smaler than the production of neoprotocorms in the culture which was illuminated with the fluorescent light.